A single layer of microvascular endothelial cells build the walls of capillaries. Molecules can diffuse through this thin layer easily. As the interface between circulating fluid in the lumen and surrounding.

Medicyte's human primary microvascular endothelial cells (mvEC) are isolated from dermis of juvenile foreskin and adult skin (different locations). By applying its proprietary and novel upcyte^® technology, Medicyte is able to extend the lifespan of human primary mvECs. Those human upcyte^® mvECs are able to proliferate while retaining their primary cell characteristics without being immortalized.

Large, virtually unlimited batches (600-800 vials) of human upcyte^® mvECs from one donor can be generated such that they retain their specific endothelial characteristics.

Upcyte^® mvECs are provided in 2 ml cryo-vials with a minimum of 5x10⁵ or 1x10⁶ viable cells. Recovery rates of approximately 70% are to be expected.

Characteristics:

• Cell morphology
• 95% positive for lecithin surface staining
• Markers: CD31⁺, vWF⁺, eNOS⁺, CD105⁺, CD146⁺, Lectin⁺, Flk-1⁺, CD34^-, Collagen I negative
• Tube formation positive
• Migration positive
• Dil-Ac-LDL uptake positive

Tested Applications:

• Angiogenesis Research
• Migration Studies
• Wound Healing
• Drug Development (Uptake Studies)
• Immunology

For each product a protocol for use and a certificate of analysis will be delivered. All offered upcyte^® cells can be used in BSL1 laboratory or higher and are tested for CMV, HIV, Hepatitis B and C.

Nevertheless, the cells - like all products of human origin - should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

For cryopreserved upcyte^® mvECs vials from our master cell bank, please inquire here.