Currently, only a few primary cell types are commercially available, and their poor proliferative capacity limits their use in research. The use of immortalized cell lines on the other hand, is compromised by their transformed, cancerous phenotype.
Using Medicyte´s innovative technologies, upcyte® and vericyte®, primary cells are driven into proliferation, thus allowing controlled and reversible bypass of cell cycle control mechanisms without inducing immortalization, uncontrolled cell growth or changing the typical phenotype.
Therefore, upcyte® and vericyte® cells are available in sufficient quantities with a consistent quality that can be used for research, screening and drug development.
Upcytes® are genetically engineered cell strains. By using a viral gene transfer system the primary cells are driven into proliferation through the introduction of proliferation genes.
We can offer you up to 2000+ vials per cell type and donor – data from different studies can now be compared while working with the same donor.
Similar sensibility to primary cells during screening phases can exclude compounds at an early stage, means toxic compounds do not reach animal testing stages. Safe life and money!
The vericyte® technology comprises proliferation factors added to the medium, which are able to induce a reversible state of proliferation in differentiated, otherwise non-proliferating primary cells. As long as the vericyte® factors are present in the medium, the cells divide. After withdrawal of vericyte® factors, the process of cell division is interrupted and the cells return to their original state.
As a result of these unique technologies, both upcyte® and vericyte® cells have a distinct advantage over commonly used cells with respect to the virtually unlimited availability of differentiated cells.
For each product a protocol for use and a certificate of analysis will be delivered. All cells have been tested for the absence of CMV, HIV, Hepatitis B and C and microbial contaminants (fungi, bacteria and mycoplasma). They can be used in BSL1 laboratory or higher.
Nevertheless, the cells - like all products of human origin - should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.